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Reagents and Materials Sterile hMSC cultures, rst or second passage DMEM-LG-10FB Adipogenic induction medium (AIM), consisting of DMEM-HG-10FB, 1 M dexamethasone, 100 M indomethacin, 500 M 3-isobutyl-1-methylxanthine (IBMX), and 10 g/ml insulin (See Section 2.5) Adipogenic maintenance medium (AMM): DMEM-HG-10FB with 10 g/ml insulin Trypsin-EDTA (See Protocol 2.4) Tissue culture dishes, 3.5 cm, or 6-well plates Protocol (a) Calculate the number of cultures needed for the experiment. (b) Trypsinize rst- or second-passage hMSCs as described in Protocol 2.4. (c) Seed cells into 3.5-cm dishes (or 6-well plates) at a density of 2 105 cells in DMEM-LG-10FB. (d) Incubate at 37 C overnight. (e) On day 1, switch medium for cultures to be induced to AIM, and replace medium in control cultures with fresh DMEM-LG-10FB. Set up the desired number of cultures for each condition. (f) Media are changed twice a week. (g) On day 10, replace AIM with AMM. c# qr code reader open sourcewindows phone 8 qr code reader c# [Solved] converting a webcam into a qrcode scanner in csharp 4.5 ...
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