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bone [Dennis and Caplan, 1993; Lennon et al, 2001] On the other hand, cartilage is more commonly observed than bone for rabbit MSCs The in vivo ceramic cube assay system is complemented by the use of a number of in vitro assays, each of which is speci c for different differentiated cells Elevated alkaline phosphatase activity, although not unique to osteogenic cells, is a useful early marker for osteogenic differentiation Accordingly, biochemical and cytochemical assays for alkaline phosphatase are used early in the culture of MSCs in osteoinductive conditions On the other hand, calcium biochemistry and von Kossa staining to detect mineralization are used later in the culture period These assays and the conditions that promote osteogenic differentiation are described in Section 7 Aggregate or pellet cultures can be established in a de ned medium to promote chondrogenic differentiation of MSCs [Johnstone et al, 1998].

I (X n ; Y n ) = H (Y n )

1.

Such cultures will not be covered in this section as they are treated thoroughly in 4 of this book Differentiation of MSCs to adipocytes can be induced through the use of a unique medium in cultures seeded at a slightly higher density than that used for osteogenic differentiation Flow cytometric analysis after Nile Red staining is used to assess expression of the adipocytic phenotype after induction This methodology is described in Section 8 The ability of MSCs to differentiate along these various phenotypic lines is strongly suggestive of their stem cell nature MSCs, although sometimes referred to with different terminology and derived from a number of species, have also been shown to differentiate into other mesenchymal lineages, including skeletal muscle [Wakitani et al, 1995], cardiac muscle [Tomita et al, 1999], and hematopoietic supportive tissue [Ko et al.

than all other types (re ecting rapid response to shallow recharge), while the lowest variability in ow rates tends to be shown by fault and doline springs arising from con ned aquifers (re ecting the generally slow response to recharge at depth). By nature, depression springs and contact springs are restricted to uncon ned aquifers (or at least to the uncon ned portions of aquifers which are elsewhere con ned). Fault springs can occur in either uncon ned or con ned aquifer settings. Where they occur in con ned aquifers, the fault zone in question must itself be permeable, thus providing a pathway to the surface for waters con ned at depth. Doline springs can also discharge

Cone-based topology control (CBTC(a)) [13] is a two-phase localized algorithm. Each node nds the minimal power p such that transmitting with the power p ensures that the node can reach some node in every cone of degree a. The algorithm has been proved to preserve network connectivity if a , 5p=6. Several optimizations to the basic algorithm have also been discussed. These include (1) a shrink-back operation can be added at the end to allow a boundary node to broadcast with less power, if doing so does not reduce the cone coverage; (2) if a , 2p=3, asymmetric edges can be removed while maintaining the network connectivity; and (3) if there exists an edge from node u to node v1 and from node u to node v2, respectively, the longer edge can be removed while preserving connectivity, as long as d(v1 , v2 ) , max{d(u, v1 ), d(u, v2 )}. As shown in Figure 10.14, the resulting subgraph may be directed. An event-driven strategy was also proposed to recon gure the network topology in the case of mobility. Each node is noti ed when any neighbor leaves/joins the neighborhood and/or the angle changes. The mechanism used to realize this requires the

RESULTS OF EXPERIMENT 1 The e ects of three di erent types of impairments, that is, packet loss, network delay, and jitter, are measured using four di erent voice clips man1p2.pcm, boy1p2.pcm, girl1p2.pcm, and wom1p2.pcm each playing the same sentence or message. The impairments are introduced separately, that is, only one type of impairment is introduced at any point in time using the NIST-Net. The results are as presented in Figures C-5, C-6, and C-7. It is clear that both packet loss and delay jitter signi cantly impair voice quality compared with network delay. As the value of delay jitter increases, the call-progress tones and speech signal become unintelligible. Also, the higher the value of network delay, the more di cult it becomes to establish a call or connection. This can be attributed to expiration of various timers during the call setup stage.