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Array: Use this option to create a blank array that you can populate with several string or numeric values Object: Use this option to create a group of objects This is similar to creating an array, but the number of objects can grow or shrink according to your needs..

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iii) Place standards on the top and bottom of the plate (A2-A9, H2-H9) iv) Wells A1 and H1 should be left empty for the blank v) Pipette 263 l 16 g/ml PG standard working stock solution to the rst well (A2, H2) vi) To the remaining wells pipette 75 l dilution buffer vii) Perform serial dilutions of 1/14 for the remaining wells by transferring 188 l with a multichannel pipette viii) 188 l of A2 is transferred to A3 and mixed, then 188 l of A3 is transferred to A4, etc A dilution of 188 l up to 263 l is a 1/14 dilution factor Final volume in all standard wells after dilution = 75 l Concentration Range of Standard ( g/ml): 16; 1143; 816; 583; 416; 297; 212 Note: The working range of the standard is only from 11.

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report many similarities in experiential, psychological, and performance states Although peak moments are quite similar to one another, there are subtle differences between them The feeling and performance model of peak moments includes two orthogonal dimensions: feeling and performance (Privette & Bundrick, 1987, 1991, 1997) As depicted in Figure 272, the feeling dimension ranges from one extreme of misery to the opposite extreme of ecstasy The performance dimension ranges from an extreme of total failure to personal best The center of the figure is neutral and typifies many everyday experiences consisting of average performance and neutral feelings The entire top right quadrant of the feeling and performance model depicted in Figure 272 contains diverse yet somewhat similar peak moments that tend to occur in physical activity.

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43 g/ml to 212 g/ml The concentration of 16 g/ml is a convenient starting dilution by which to obtain the working range and should not be used in calculations because it is off scale Sample Dilution Scheme i) Pipet 150 l diluted sample into the rst well ii) To the remaining wells pipet 75 l dilution buffer iii) Perform serial dilutions of 1/2 by transferring 75 l; 75 l of B1 is transferred to B2 and mixed, then 75 l of B2 is transferred to B3, etc A dilution of 75 l up to 150 l is a 1/2 dilution factor, and the nal volume in all sample wells after dilution is 75 l..

Peak Performance A peak performance is spontaneous, represents superior functioning, reflects a high standard of accomplishment, and can result from a wide variety of activities Interestingly, participation with others, as occurs in team games, may hinder the likelihood of experiencing a peak performance Being with others may disrupt the exerciser s concentration (Privette & Landsman, 1983) Examples of exercise- and sport-related peak performances are a hole in one in golf, a perfect serve in tennis, and an effortless 10mile jog for a runner who usually is exhausted after jogging 5 miles A peak performance promotes strong perceptions of competence, excellence, mastery, and self-efficacy that can enhance quality of life Such perceptions tend to promote feelings of satisfaction and well-being that are crucial to quality of life (Berger et al, 2007) Peak Experience A peak experience ref lects a psychological state that includes intense joy and happiness.

It can be any experience that evokes strong, positive affective states such as peace, great joy, illumination, loss of self, or ecstasy (Privette & Bundrick, 1987) Peak experiences are memorable, fulfilling, and personally meaningful moments that affect the quality of life (Maslow, 1968) An example of a peak experience in the realm of exercise for one individual might be jogging on a deserted beach, experiencing intense joy while watching a beautiful sunset, and hearing the seagulls calling to one another overhead (eg, Battista, 2004, pp 23 28; Berger et al, 2007, p 44) Peak experiences have been reported by exercisers who swam the English Channel; reflecting on the personal meaning of finishing the swim, they reported.

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